Mandai Nature is jointly established by Temasek and Mandai Park Holdings. It is established in Singapore as a non-profit organisation and an Institution of Public Character. Mandai Nature is dedicated to protecting threatened species, nurturing healthy ecosystems and creating vibrant communities where wildlife and people can thrive and co-exist, in Singapore and Southeast Asia. Through collaborations with like-minded partners, Mandai Nature aims to protect threatened species from extinction, especially those endemic to Asia and often overlooked, including addressing issues of wildlife trade and the fragmentation of habitats. It drives nature-based solutions for climate change focusing on green and blue carbon including protecting and restoring tropical forests, mangroves and peatlands, while working closely with local communities and organisations to create economic opportunities and invest in building skills and conservation capacity on the ground.

Mandai Nature's Singapore Program aims to contribute towards enhancing Singapore's efforts to support biodiversity conservation in Singapore and making links with the broader region. The programs will focus on specific areas such as protection of endangered species, such as the critically endangered Sunda Pangolin, urban wildlife co-existence, climate change mitigation and adaptation, and capacity building for young scientists.